A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm
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چکیده
منابع مشابه
X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.
Gram-positive bacteria possess a thick cell wall composed of a mesh polymer of peptidoglycans, which provides physical protection. Endolysins encoded by phages infecting bacteria can hydrolyse peptidoglycans in the bacterial cell wall, killing the host bacteria immediately. The endolysin (Psm) encoded by episomal phage phiSM101 of enterotoxigenic Clostridium perfringens type A strain SM101 exhi...
متن کاملTitle : Lytic activity of secreted LysH 5 endolysin by Lactococcus lactis using the 1 secretion signal
19 20 Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin 21 LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed 22 and secreted in Lactococcus lactis using the signal peptide of the bacteriocin lactococcin 23 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of 24 extracelullar active endolysin was detected i...
متن کاملA Thermophilic Phage Endolysin Fusion to a Clostridium perfringens-Specific Cell Wall Binding Domain Creates an Anti-Clostridium Antimicrobial with Improved Thermostability
Clostridium perfringens is the third leading cause of human foodborne bacterial disease and is the presumptive etiologic agent of necrotic enteritis among chickens. Treatment of poultry with antibiotics is becoming less acceptable. Endolysin enzymes are potential replacements for antibiotics. Many enzymes are added to animal feed during production and are subjected to high-heat stress during fe...
متن کاملStructure-based modification of a Clostridium difficile-targeting endolysin affects activity and host range.
Endolysin CD27L causes cell lysis of the pathogen Clostridium difficile, a major cause of nosocomial infection. We report a structural and functional analysis of the catalytic activity of CD27L against C. difficile and other bacterial strains. We show that truncation of the endolysin to the N-terminal domain, CD27L1-179, gave an increased lytic activity against cells of C. difficile, while the ...
متن کاملRapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin.
OBJECTIVES Streptococcus pyogenes, or Group A streptococcus (GAS), has a propensity to colonize human tissues and form biofilms. Significantly, these biofilms are a contributing mechanism of antibiotic treatment failure in streptococcal disease. In this study, we evaluate a streptococcal-specific bacteriophage-encoded endolysin (PlyC), which is known to lyse planktonic streptococci, on both sta...
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ژورنال
عنوان ژورنال: Antibiotics
سال: 2021
ISSN: 2079-6382
DOI: 10.3390/antibiotics10030245